INSULIN STIMULATES AUXIN-DEPENDENT CELL PROLIFERATION IN NT-1 TOBACCO SUSPENSION CELL CULTURES
DOI:
https://doi.org/10.5377/nexo.v29i2.4572Keywords:
Insulin, auxin, proliferation, NT-1 cells.Abstract
Insulin regulates growth in plants such as maize and beans, through cell proliferation. In this study we observed that in NT-1 tobacco cell cultures, the hormone regulates cell proliferation dependent on the presence of auxins. The cultures where the auxin concentration was limited showed a low mitotic activity, which was reestablished by the addition of 1.23 nM of insulin through the expression of the cyclin B, CDKB, E2F, G protein subunit β ARC A and glutation -S-transferase (GST) genes. The cultures also presented an increase in dry weight and small cells corresponding to a phenotype with high cell division. On the other hand, it has been reported that the absence of auxins in this type of culture of suspended tobacco cells leads to a partial synchronization, and in this system insulin also promoted proliferation, increasing the expression of the E2F gene.Downloads
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Published
2016-12-31
How to Cite
Fierros-Romero, G., Mellado-Rojas, M. E., & Beltrán-Peña, E. M. (2016). INSULIN STIMULATES AUXIN-DEPENDENT CELL PROLIFERATION IN NT-1 TOBACCO SUSPENSION CELL CULTURES. Nexo Scientific Journal, 29(2), 44–58. https://doi.org/10.5377/nexo.v29i2.4572
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