Production of potato microtubers (Solanum tuberosum L.), Variety Burren in temporary immersion biorreactors

Abstract

The study microtuberización with potato cultivar Burren was conducted in tissue culture laboratory of the National Agrarian University between June and December 2015. Phases of multiplication and formation of microtubers were studied in bioreactors Economic Temporary Immersion (BEIT). The best variants of culture media were those containing 0.20 mg l-1 GA3 with 0.50 mg l-1 BAP and 0.10 mg l-1 GA3 with 1 mg l-1 BAP, with average respective number of inter- nodes 6.32 and 5.7 and number of leaves per plant of 7.37 and 6.93. Additions sucrose 80 g l-1 and 110 g l-1 favored the formation of microtubers with average fresh weight between 0.69 and 0.75 grams. Sucrose concentrations of 80 g l-1 and 120 g l-1 were no significant statistical differences between the means of the variable diameter and length of microtubers were recorded. In microtubers more than 10 mm diameter averages obtained plant length , number of leaves and number of buds of 21 cm , 16.35 and 5.65 respectively and with diameters between 8 and 10 mm the respective means were 16.37 cm , 11.35 and 5.35. In microtubers more to 10 mm diameter averages obtained plant length, number of leaves and number of buds of 21 cm, 16.35 and 5.65 respectively and with diameters between 8 and 10 mm the respective means were 16.37 cm, 11.35 and 5.35.