Identification of Lactobacillus sp with probiotic potential from fermented yuca (Manihot esculenta)

Abstract

In Nicaragua systems of production cattle they are aff ected by the low availability of food and water in dry season causing the increase of diseases and malnutrition, aff ecting directly the productive and reproductive herd parameter, Likewise counteract this types of situations comes the of antibiotics by prophylactic therapeutic use and growth promoters, able to eliminate pathogens and eliminate drastically on the microbial fl ora necessary for the functioning or the digestive system also to infl uencing the accumulation of waste in fi nal products of importance to consumers, the genus lactobacillus represents a high biotechnological potential, this bacteria contribute to the increase organoleptic of food and generate an unfavorable environment for the growth of pathogens microorganisms. The objective of this work was identify with vitro test the species of lactobacillus with high probiotic through fermented substrate of yucca, through biochemical tests, lactobacillus sp, L.casei and L.brevis was identifi ed, the lactobacillus species previously identifi ed was in a resistance tests with diff erent level of pH showing resistance to the acid conditions, the microbial antagonism test in front E.coli ATCC 25922 and salmonella sp demonstrate the presence of inhibition of halos, showing antimicrobial action over the pathogens growth the L.casei and L.brevis species was sensitive to Trimetoprim sulfametaxona and oxitetraciclina antibiotics, to realize the sensitive tests. These results demonstrate that the identifi ed strains can be used as a probiotic in the feeding of the animals or as an inoculant in the silage process.