Comparison between the smears of central blood and peripheral blood for the diagnosis of hemoparasites in bovine

Abstract

Faced with disagreements between the clinical and laboratory diagnosis, it was decided to make a comparative study between the diagnostic study of central and peripheral blood samples, to compare results. 100 cows were sampled from ten diff erent farms (10 cows from each farm) in the town of Pancasan, Matagalpa, Nicaragua, extracting from each one samples of central blood (jugular vein) and peripheral blood (auricular vein). All the samples were processed by means of a thin smear, thick smear and capillary visualization (Woo technique), dyeing them both with the Diff Quick dyes and with Giemsa. The central or peripheral blood tests have very large errors, greater than 20%, as to fully rely on only one of them in the results of studies on hemoparasites. Peripheral blood is only more eff ective in diagnosis at 2.79%, which is not signifi cant (P <0.1). It is recommended to carry out both simultaneous analyzes (central and peripheral blood) for future diagnoses of haemoparasites in the laboratory.