Effect of APH treatment on the preservation of mango fruit (Mangifera indica) Kent variety

Abstract

The present experimental research study was carried out with the purpose of determining the growth, inhibition or destruction of pathogens on days 0 (t₀), 7 (t₇), 15 (t₁₅) and 30 (t₃₀); by treatment of HHP 400 and 500 MPa, using the method of quantification or counting of cfu colony-forming units, on three different cocktails of bacteria, such as Listeria monocytogenes, Escherichia coli and Salmonella, the microbial contained in the epicarp or peel of the mango fruit was also investigated. The results in this study show that the application of high hydrostatic pressures, with a treatment of 400 MPa of pressure 8 min of duration, managed to eliminate a load of 5.16 Log, cfu count / gr of the day t₀, under conditions of 20 ºC of temperature, on sample of Mangifera indica, var. Kent, with a pH of 3.4, on L. monocytogenes under the same conditions was performed in treatment of 500 MPa of pressure with 8 min of time, having a reduction of 4.64 Log, in a t₀, HHP treatments was efficient reducing 6 Log, for t₇, t₁₅ t₃₀. Similarly, an inactivation of Salmonella was achieved in t0, for HHP 400 MPa 7.74 Log was eliminated, and in 500 MPa 6.84 Log, for a pure culture with a control load of 9.14 Log, it was observed that, in the case of treatments, they do not present bacterial growth, as several authors argue, causing death by cell damage at times t₇, t₁₅, t₃₀.  Likewise, E. coli was studied, for t₀, there was a reduction in HHP treatments of 400 MPa of 5.64 Log, for HHP 500 a reduction of 5.04 Log, it was observed that in the treatments there was cell damage in t₇, t₁₅ and t₃₀ existing a total reduction, it is concluded that in the case of E. coli according to the data obtained there was a total reduction of bacteria. The microflora of the mango skin was studied, it was established that there was a reduction of 3 Log, on average general for treatments of 400 and 500 MPa for times t₀, t₇, t₁₅ and t₃₀, observing a resistance for both treatments, due to the presence of yeasts.