In vivo effect of alcohol on ATPase activities in rat organs

Abstract

There are enzymes that hydrolize ATP in membrane cells of several tissues: the Na, K ATPase and the Na ATPase. The first is fundamental for life, keeping Na+ and K + gradients through cell membranes, something needed for a great quantity of cell functions. The second is involved in active regulation of the cell volume. OBJECTIVES: To determine in vivo effect of alcohol (ethanol) on the activity of these enzymes, in order to know if this can affect the functioning of the organism. To determine if the alteration occurs in several organs and if it is reversible. MATERIALS AND METHODS: Organs homogenates from rats subjected or no to alcoholic diet, were prepared and assayed for their ATPase activities. RESULTS. It was found that ethanol, in vivo, causes a stimulatory effect on Na- ATPase activity, without showing any effect on Na,K-ATPase activity; this occur in several organs and was reversible. CONCLUSIONS: The stimulation of the alcohol intake on the activity of the Na-ATPase occurs in several organs and could result in serious problems for the cell function and the organism. This activation of the enzyme is reversible.