Detection of BLA genes in clinical isolates of escherichia coli from patients from Hospital Nacional Zacamil de El Salvador
DOI:
https://doi.org/10.5377/creaciencia.v10i2.6031Keywords:
Escherichia coli, El Salvador, Infection Control.Abstract
The production of inactivating enzymes type Beta-lactamases is one of the most important mechanisms of antimicrobial resistance reported worldwide. Infections caused by microorganisms producing extended-spectrum Beta-lactamases are associated with higher mortality. Because of the ability to spread through transposable elements, bla genes are widely reported. However, there are countries with no reports of their circulation, despite their clinical importance.
This study provides the first evidence of Gram-negative in-hospital infections associated with Beta-lactamase genes in El Salvador. Objective: Determine the presence of bla genes type TEM, SHV, CTX-M1-2 and AmpC in Escherichia coli clinical isolates patients at Hospital Nacional Zacamil. Methods: Thirty consecutive Escherichia coli isolates from patients were studied from January to May 2015. Species confirmation and resistance determination were performed with PromptTM Inoculation Sistem-D for MicroScan® equipment. ESBL production was determined by double-disc synergistic method. The presence of bla genes was verifed by Chain Reaction of Polymerases. Results: Twenty-four samples were positive for ESBL determined by Double Disc method and 20 by MicroScan® automated method. The double-disc method for the phenotypic determination of ESBL production is more effective. Ciprofloxacin and ceftriazone were the most commonly used antibiotics as treatment. Conclutions: The 36.6% of isolates tested positive for bla genes type TEM. Negative results were obtained for the other genes studied.
CREA CIENCIA Vol. 10 No 2 ISSN1818-202X julio-diciembre 2016, p. 6-18
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