Amibas of free life in pools, pools and lakes of El Salvador

Abstract

In this work, 60 water samples from 30 sites in the Salvadoran territory where water reservoirs used by the population for fun are located.
The identification of each sample included: date of collection, location of the site, surface or depth, and chlorinated or unchlorinated condition.
Each sample was sedimented and this was studied between foil and lamella, using 10X and 40X microscope objectives, looking for mobile forms of amoebas.
Additionally, four agar or agar plates seeded with live or dead Escherichia coli were seeded from the sediment.
Cultures were examined using 4X objective of the microscope. Fresh preparations were made from the colonies and observed under a microscope with 10X and 40X objectives.
Preparations that showed amoeba trophozoites were fixed with PVA (polyvinyl alcohol) and stained with trichrome staining. AVL (free-living amoeba) were considered trophozoites that had two or more of these characteristics: size from 13 to 30 micrometers, rounded pseudopods, and nucleus with a prominent cariosome surrounded by a clear halo.
AVLs were found in two water samples taken from the bottom of pools with chlorinated water, and in a sample taken from the bottom of another pool of non-chlorinated water.