Efficiency of the basal nutrient media: solid and liquid in the stage of in vitro establishment of the orchid "Tripita" Trichopilia tortilis Lindl
DOI:
https://doi.org/10.5377/payds.v5i0.5429Keywords:
Orchids, growth media, germination, protocorms, imbibition, vitro-plants.Abstract
The research was based on determining the efficiency of the basal nutrient media (solid and liquid) for the in vitro establishment of the "Tripita" orchid T. tortilis Lindl., Through the number of germinated seeds, protocormos formation and development Of vitro plants.
An immature fruit (green capsule) was used, seven months after flowering; Disinfected in a soapy solution containing three drops of Tween 20, 10% sodium hypochlorite and 190 ml of distilled water. The seeds were inoculated by taking a 1ml aliquot which was deposited in each glass jar; For both nutritional media the same amount (1ml) was added with 25 replicates per treatment. Data collection was based on a completely randomized design, to which analysis of variance was applied.
Producción Agropecuaria y Desarrollo Sostenible, Vol. 5, 2016: 43-57
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