In vitro multiplication of pineapple MD-2 (Ananas comosus (L.) Merril) using commercial fertilizers

Abstract

This study consisted of an in vitro multiplication of MD -2 pineapple (Ananas comosus (L.) Merril ) under the technique of plant tissue culture, using the culture developed by Murashige & Skoog (1962 ), modified with 3mg 6 benzyl purine amino per half liter, treatment one ( T1 ) . This treatment has been contrasted with another one that is based on an in vitro culture, based on water-soluble commercial fertilizer , culture that was identified as «UNICAES 2» treatment two ( T2 ); trying to match the concentration and availability of each needed element for proper plant development .

Apical buds were cut from the base of the plant, and disinfected with 5 % sodium hypochlorite for 15 minutes, adding 3 drops of Tween 80 per 100 ml of disinfectant solution. The apical buds of approximately 0.5cm long by 0.5cm diameter were cut. These were inoculated in glass bottles containing 30ml of culture with 20 replications per treatment. The buds were incubated at 16/08 hours (light-dark); with an average temperature of 28°C and 232 foot-candle of light intensity, provided by three fluorescent lamps. After 40 days the buds obtained in both treatments, were compared in length and number of lateral buds.