Isolation and Identification of native microorganisms with potential probiotic in the shrimp (Litopenaeus vannamei) farmed in Honduras, 2018

Abstract

The use of probiotics becomes more common every day in aquaculture productions, for the prevention of diseases as the improvement of water quality and funds of production systems, minimizing the indiscriminate use of antibiotics, like improving the environment. These probiotics must be adapted to the medium that is why often we cannot see favorable short-term results. The objective was the isolation and identification of native microorganisms with potential probiotic present in the white shrimp Litopenaeus vannamei, these native microorganisms will allow us to optimize the adaptation time as they will not have to adapt because they will live in their native environment. This is a descriptive, experimental investigation; simple random sampling was done by random selection of 60 shrimp from which the gastrointestinal tract (GIT) was extracted and macerated to be planted in the selective culture media Thiosulfate Citrate Biles Sucrose Agar (TCBS), Man Rogosa and Sharpe (MRS); Potato dextrose Agar (PDA), Isolation of Actinomycetes Agar and Müeller Hinton like a non-selective culture media. The microorganisms that were isolated and identified were cultured in a bioreactor to identify the principal characteristics like fast growing and easy culture. The microorganisms that were identified were Bacillus subtilis and Actinomycetes, they have characteristics like potential probiotics. These microorganisms have the characteristics to be undemanding and with excellent growing.