Forensic parameters of ten X chromosome microsatellites polymorphisms in a sample of the Zulia State population, Venezuela

Abstract

Background: The Short Tandem Repeats are distributed throughout the human genome, those located in the autosomal chromosomes and Y chromosome have been widely used in forensic genetics laboratories due to their characteristics and hereditary patterns. Objectives: In order to characterize and determine forensic parameters of STR sequences of the X chromosome (DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08 and DXS7423) in the Zulia State population. Methodology: 108 individuals (130 X chromosomes) were selected and their DNA was amplified using Polymerase Chain Reaction technique. The amplified fragments were separated by capillary electrophoresis and the alleles were assigned by comparison with the allelic ladder. Results: The average diversity was 0.7252, the Polymorphics Information Content proved to be greater than 0.5 in all the X-STRs and the cumulative discrimination power was 0.99999997 in women and 0.99999816 in men. Conclusions: The obtained data showed that the analyzed X-STRs are sufficiently informative as to be used in cases of complex biological links and human identification.