FcyRIIA-131 associated with severe cases of dengue in Honduras

Abstract

Dengue is a disease of worldwide importance. To understand more about the pathogenesis of dengue the interest in the search for genetic factors in the host has recently increased.

We investigated whether single nucleotide polymorphisms (SNPs) in genes coding for DC-SIGN, Fc receptor (FcyRIIA) and vitamin D receptor (VDR) are associated with severe dengue and whether in this study group the severity of dengue is associated with secondary infections. A total of 200 cases of dengue patients were investigated in Tegucigalpa, Honduras, 100 cases of classic dengue and 100 cases of dengue hemorrhagic classified according to 2007 WHO criteria.

Genotyping was performed using PCR-RFLP and additionally this technique was compare to the ¡PLEX assay (SEQUENOM ® Inc. USA). A significantly increased risk of severe disease was observed in FcyRIIA in carriers of genotype CC versus TC (Chi2 = 5.29, P = 0.02, OR = 2.22). The G allele variant of the DC-SIGN1-336 was not associated with severe dengue disease. The VDR genotype frequency of CC genotype compared whit CT scan shows a tendency to increase in severe forms compared to mild forms of the disease (Chi2= 3.36, P= 0.06, OR= 0.35). These preliminary data provide new insights into the role of genetic factors in dengue; knowledge of more SNPs associated with dengue will provide information on possible mechanisms of pathogenesis and protection.

DOI: http://dx.doi.org/10.5377/rct.v0i13.1714

Revista Ciencia y Tecnología, No. 13, diciembre 2013: 67-80