Evaluation of in vitro induction media to plant callus in foliar explants of Annona diversifolia Safford (Magnoliales: Annonaceae)

Abstract

In order to induce the formation of in vitro plant calluses in foliar explants of Annona diversifolia (A. diversifolia), the use of six plant callus induction treatments in explants of three-year-old adult specimens from nursery conditions was evaluated (CAPOSA SA. de C.V.). The incubation was in darkness conditions at a temperature of 24-26°C. The experimental design was completely randomized with three repetitions of ten experimental units per treatment. No callus formation was induced in any treatment. The percentages of contamination (PC), oxidation (PO) and survival (PS), were measured weekly during a period of five weeks. The PO and PS, show significant differences at the level of 1% (F> 5.064). The treatment f (100% B5 medium (Gamborg et al., 1968) + 2,4-D (2 mg / L) + Kin (1 mg / L)) based on Gómez et al. (2006), obtained a PO of 53.33% and a PS of 46.66% during week five. The results indicate that the treatment was the most adequate to prolong survival in laboratory conditions.