Induction to the latency rupture in seeds of Trema micranthum, (Roem. & Schult.) Blume, an emerging species for the production of amate paper

Abstract

The objective of this work was the evaluation of the viability percentage in Trema micranthum seeds, which were divided into: imbibition and tetrazolium tests for the identification of viable seed for germination and no viable for germination seeds, as well as the evaluation of two promoters Germination to inhibit latency present in seeds. Seed permeability was evaluated in batches of 100 with three replicates; viability was determined by tetrazolium staining at 0.5 and 1% at different times in which the percentage of viable and non-viable seeds was obtained. On the other hand, two promoters of germination (N03) and gibberellic acid (GA3) were evaluated at three different concentrations (500, 1000 and 2000 mg L-1) with three replicates each followed by sowing in RAS medium. Finally, with the tests carried out, the percentage of viable and no viable seeds for germination seeds was determined.  The seeds were used in the evaluation of the permeability of the demonstration that after 10 hours reached a maximum of the water absorption, the determination of the seed show had the main result with the tetrazolium 1.0%. Evaluation of the two promoters resulted in approximately 33% germination with GA3 [500 mgL-1] and 18% with KNO3 [500 mgL-1]. Finally, tests that were carried out on 50% viable seeds 28% viable non-germinable seeds, 22% germinable and these results were obtained by staining tests and in the evaluation of the two germination promoters,